Generate the best possible toehold switches to detect your desired RNA transcripts.
Customize your RBS, promoter, RE sites, and temperature.
Remove unwanted stop codons.
Limit the number of base pairs on the toehold domain.
Generate triggers to test your switches.
Compare free energies and secondary structures of possible duplexes and RBS-linker sequences.
Generate primers to synthesize and clone your switch into plasmids.